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Image Search Results
Journal: Biochimica et Biophysica Acta
Article Title: cAMP inhibits migration, ruffling and paxillin accumulation in focal adhesions of pancreatic ductal adenocarcinoma cells: Effects of PKA and EPAC
doi: 10.1016/j.bbamcr.2013.06.011
Figure Lengend Snippet: Increasing cAMP inhibits migration of PANC-1 cells. (A) PANC-1 cell migration, as measured by Boyden chamber assays (n = 18 for each condition), was significantly inhibited by 20 μM forskolin (Frsk), 1 mM IBMX and a combination of the two reagents. (B) The effect of 20 μM Frsk and a combination of 20 μM Frsk/1 mM IBMX on cytosolic cAMP levels in PANC-1 cells (n = 34). (C) The effect of 1 mM IBMX and a combination of 20 μM Frsk/1 mM IBMX on cytosolic cAMP levels in PANC-1 cells (n = 54). (D) PANC-1 cell migration, as measured by Boyden chamber assays (n = 9 for each condition), was significantly inhibited by 8Br-cAMP. (E) The effect of 8Br-cAMP on cytosolic cAMP levels in PANC-1 cells (n = 37); 20 μM Frsk and 1 mM IBMX were added at the end of experiments to saturate the probe. (F) PANC-1 cell invasion, as measured using Matrigel-coated Boyden chambers (n = 6 for each condition), was inhibited by 20 μM Frsk and 1 mM IBMX.
Article Snippet: To test cell invasion,
Techniques: Migration